| Unlockable: | How to Unlock: |
| Agent Muriel Green | Successfully complete The Den of Souls on the Hard difficulty setting |
| Antadurunnu | Successfully complete The God Seal on the Hard difficulty setting |
| Arnold Leech | Successfully complete Al Khali on the Easy or Normal difficulty setting |
| Bishop Malthus St. Claire | Successfully complete Crusades on the Easy or Normal difficulty setting |
| Blind Behemoth | Successfully complete Morituri te salutant on the Hard difficulty setting without being incapacitated |
| Brother William of Auxere | Successfully complete Motley Crew on the Hard difficulty setting |
| Centurion Textius Longinus | Successfully complete Decadence on the Hard difficulty setting without being incapacitated |
| Child Crusader | Successfully complete Crusades on the Easy or Normal difficulty setting |
| Corpses Behemoth | Successfully complete River of Blood on the Hard difficulty setting |
| Cpl. Simone Cole | Successfully complete The Tomb on the Hard difficulty setting |
| Cpt. Devin Ross | Kill 50 enemies using melee attacks |
| Cpt. Xavier Jones | Successfully complete Man Down on the Hard difficulty setting |
| Crossbowman Crusader | Successfully complete Tortured Souls on the Hard difficulty setting |
| Cultist | Kill 100 enemies using melee attacks |
| Deceased Devin Ross | Successfully complete Still With You on the Hard difficulty setting |
| Enlil | Successfully complete Spiritual Guide on the Hard difficulty setting |
| Exlposive Cultist | Disintegrate 50 enemies |
| Flamethrower | Disintegrate 100 enemies |
| Fr. Paul Rawlings | Pop 100 heads |
| Gladiator | Successfully complete Temple of Pain on the Hard difficulty setting |
| Governor Cassus Vicus | Successfully complete Rome on the Easy or Normal difficulty setting |
| Grenadier | Kill 100 enemies using Ghost Bullet |
| Inanna | Successfully complete Skin on the Hard difficulty setting |
| Ki | Successfully complete Flesh on the Hard difficulty setting |
| Legionary | Successfully complete The Low Road on the Hard difficulty setting |
| Lt. Abigail Black | Kill 50 enemies using Ghost Bullet |
| Nanna | Successfully complete Blood on the Hard difficulty setting |
| Ninlil | Successfully complete Skin on the Hard difficulty setting without being incapacitated |
| Operation: Godseal | Successfully complete Exorcism on the Hard difficulty setting |
| Psychic Commander | Successfully complete World War 2 on the Easy or Normal difficulty setting |
| Pyxis Prima | Successfully complete Sumeria on the Easy or Normal difficulty setting |
| Sgt. Frank Delgado | Pop 50 heads |
| Sgt. Wilhelmina Billie Church | Successfully complete Al Khali on the Hard difficulty setting |
| Sir Richard de Gray | Successfully complete Out of the Frying Pan on the Hard difficulty setting |
| Sumerian Puppet | Successfully complete Flesh on the Hard difficulty setting without being incapacitated |
| The Jericho Team | Successfully complete Rivers of Blood on the Hard difficulty setting without being incapacitated |
| Utu | Successfully complete Blood on the Hard difficulty setting without being incapacitated |
| Warrior Crusader | Successfully complete Sewers on the Hard difficulty setting |