| Unlockable: | How to Unlock: |
| A Dark Reward (20 Pts) | Secret Achievement |
| Aleksei Sytsevich (30 Pts) | Defeat this nemesis. |
| Arachnophile (10 Pts) | Collect all Secret tokens. |
| Bomb Squad (20 Pts) | Get at least Bronze on all bomb tours. |
| Bronze Finalist (20 Pts) | Get at least Bronze on all trick races. |
| Cold Blooded Super Master (30 Pts) | Complete all Connors missions. |
| Collateral Damage (10 Pts) | Destroy 25 objects. |
| Curtis Connors (30 Pts) | Defeat this nemesis. |
| Deputy (30 Pts) | Complete all DeWolfe missions. |
| Eddie Brock (40 Pts) | Defeat this nemesis. |
| Fast Swinger (20 Pts) | Swing at 200 miles per hour. |
| Fire Breather (10 Pts) | Collect all Order of the Dragon Tail tokens. |
| Flint Marko (30 Pts) | Defeat this nemesis. |
| Frequent Flyer (10 Pts) | Collect all Skyscraper tokens. |
| Gold Medal Winner (30 Pts) | Get Gold on all trick races. |
| Harry Osborn (30 Pts) | Defeat this nemesis. |
| Hitch Hiker (20 Pts) | Ride a car or truck for 5 miles. |
| Intermediate Racer (20 Pts) | Get at least Bronze on all easy and medium trick races. |
| Luke Carlyle (30 Pts) | Defeat this nemesis. |
| Macdonald Gargan (40 Pts) | Rescue this adversary. |
| Mary Jane Thriller (20 Pts) | Complete all Mary Jane thrill rides. |
| Master Tourist (20 Pts) | Take a 500-point photograph. |
| Master Web Swinger (20 Pts) | Swing a total of 50 miles. |
| Mega Tourist (10 Pts) | Take a photograph from a great height. |
| Mega Web Swinger (30 Pts) | Swing a total of 200 miles. |
| Pied Piper (10 Pts) | Collect all Subway tokens. |
| Pole Swinger (10 Pts) | Swing on 50 poles. |
| Police Chief (30 Pts) | Complete all combat tours. |
| Police Corporal (30 Pts) | Complete all Petty Theft missions. |
| Police Detective (30 Pts) | Complete 25 random city missions. |
| Police Officer (30 Pts) | Complete all Crime Spree missions. |
| Police Sergeant (30 Pts) | Complete all Bank Robbery missions. |
| Punk Rocker (10 Pts) | Collect all Apocalypse Gang tokens. |
| Sergei Kravinoff (30 Pts) | Defeat this nemesis. |
| Shutterbug (20 Pts) | Complete all Daily Bugle photo missions. |
| Silver Finalist (20 Pts) | Get at least Silver on all trick races. |
| Spider-Man (50 Pts) | Complete all missions. |
| Splat Master 2007 (20 Pts) | Splat into the ground 25 times. |
| Sweet Tooth (10 Pts) | Collect all Arsenic Candy tokens. |
| Trick Novice (20 Pts) | Get at least Bronze on all easy trick races. |
| Web Slinger (20 Pts) | Web up 25 enemies. |
| Web Swinger (20 Pts) | Swing a total of 10 miles. |
| Wilson Fisk (30 Pts) | Defeat this nemesis. |